- Human Serum off-the-clot, Type AB, male, sterile filtered
- Product Number : P40-2701
- Size : 100 ml
- Store at : -20°C
- Sterile : Yes
- HS-Code : 30021200
- Human Serum off-the-clot, Type AB, male, sterile filtered
- Product Number : P40-2702
- Size : 500 ml
- Store at : -20°C
- Sterile : Yes
- HS-Code : 30021200
- PAN-Biotech is known as a renowned producer of sera for cell culture. Our customers benefit from our years of experience and highly specialized products.
- From collection to the final product
+ Human Sera is the future for human cell therapy.
+ Therefore the highly sensitive material and is subject to stringent safety EU regulations.
+ The sample collectionis taken from human, healthy donors in Europe, certified donations institutions.
+ The average age of our donors is approximately 26 years.
+ The collection and testing of human plasma is carried out in accordance with the current version of Good Manufacturing Practice Annex 14th.
+ Each donation is precisely tested regarding various biophysical parameters and viral infections and negative in the tests anit HIV 1/HIV 2, Hbs Ag, anti-HCV, HIV-1 Ag and/or HIV-1 NAT and Syphilis.
+ From the collection of the plasma up to the packaging of the final product each individual production step is controlled and documented.
+ Therefore, we provide our customers characterized with sera of consistently high quality, an absolute maximum level of safety and transparency.
+ In each batch of human serum among others the osmolarity, pH, protein- and glucose concentration, and cell growth are measured and the sterility tested.
+ Human Sera produced by PAN-Biotech consist exclusively of human material.
+ Human serum may be more suitable for the cultivation of human cells than FBS since serum and the cultured cells belong to the same species.
+ Thus, the physiological and natural conditions in vitro are reproduced most similar to those in vivo.
+ Human serum is particularly suitable for cell culture of human cells, tissue engineering of human tissue, human cell therapies (Clinical Trials I and II), particularly sensitive cell lines and immune cells.
+ PAN-Biotech offers three different categories of human serum: "Off the clot", Seraclot and converted human serum.
+ Within these three categories it is possible to choose between a mixed groups pool or human serum AB.
- Off-the-clot serum
+ Off-the-clot serum is prepared from human whole blood collected without anti-coagulant, allowed to clot at room temperature and then centrifuged to remove the clot.
+ We provide single donor units as well as pooled off-the-clot serum.
+ Off-the-clot serum is filtered through depth and membrane filters before filling.
- Human Serum from PAN-Biotech
+ Human serum is produced from human plasma by addition of calcium chloride.
+ This results in clotting of the plasma.
+ After the coagulation process the cellular and solid constituents of the serum are removed by centrifugation, the human serum is washed and concentrated by ultra-filtration and finally filtered through a combination of depth- and membrane- filters.
+ PAN-Biotech offers also converted human serum from donors with the blood group AB.
+ Their blood does not contain anti-A nor anti-B antibodies, that could damage cultured cells and is therefore particularly suitable for immunological and hematopoietic studies.
- Customer information
+ Human serum is a natural product. After the sterile filtration it is bottled and frozen as a clear solution. After thawing a slight blur may occur.
+ Thus does not mean any loss of quality and the serum can be added to the medium without concerns.
+ Human serum is usually used in a concentration between 5% and 20%.
+ Cells cultured with FBS should be adapted step by step to the new serum.
+ Initially, the cells should be cultivated for 4-6 days with a 10% serum mixture, consisting of 5% FBS and 5% human serum.
+ Afterwards the final conversion to human serum can take place
