<p style="text-align:justify">The main purpose of buffered salt solutions is the maintenance of pH and osmolarity in cell culture applications as well as providing essential inorganic ions. These solutions are usually used for washing tissues and cells. Often they are combined with other agents in cell and tissue treatment. Alsever's Solution is an isotonic balanced solution. It is used as an anticoagulant or blood preservative. Whole blood can be stored at refrigerator temperature up to 10 weeks. In normal whole-blood erythrocytes are thousand times more frequently than leucocytes are and show an impediment for investigation or analysis of leucocytes. Erythrolyse buffers or Red-Cell-Lysing-Buffer is intended for the lysis of red blood cells in samples with white blood cells as for example EDTA-/citrate/heparin-treated whole-blood, Buffy Coats (PBMCs, leucocyte films), bone marrow. Red-Blood-Cell-Lysis-Buffer is specially used for lysing human erythrocytes in single-cell suspensions of peripheral blood and mouse hematopoietic tissues such as spleen. This buffer contains ammonium chloride, which lyses red cells, with minimal effect on lymphocytes when used as instructed. Nucleated red cells are not effectively lysed with ammonium chloride.</p>
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