- PRODUCT DESCRIPTION
+ The ready to use system is designed for all applications required for Real-Time PCR.
+ Hot-start Taq DNA polymerase is activated by heating up the reaction 10 min at 95 oC.
- PRODUCT SPECIFICATION
+ During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end,
+ Separates the reporter dye from the quencer dye only when the probe hybridizes perfectly to the target DNA.
+ This cleavage results in the fluorescent signal which is monitored by Real-Time PCR detection system.
+ An increase in the fluorescent signal (CT) is proportional to the amount of the specific PCR product.
+ HotStart Taq DNA Polymerase enzyme contains Anti-Taq monoclonal antibodies to ensure specificity and thermostability.
+ Real Time Universal Master Mix contains 0.2 mM of each dNTP, 3 mM MgCl2, HotStart Taq DNA Polymerase and ROX passive reference dye
