- PRODUCT DESCRIPTION
+ SNP Fish Species Detection for Sea Bream Real Time PCR Kit is used for the detection of Sea Bream (Sparus aurata) DNA in raw, processed food and fodder.
+ The Kit provides high sensitivity with a limit of detection (LOD) below the officially required level of 0.1%.
- PRINCIPLE OF THE SYSTEM
+ During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end,
+ Separates the reporter dye from the quencer dye only when the probe hybridizes perfectly to the target DNA.
+ This cleavage results in the fluorescent signal which is monitored by Real-Time PCR detection system.
+ An increase in the fluorescent signal (CT) is proportional to the amount of the specific PCR product.
