- PRODUCT DESCRIPTION
+ Congenital deafness is inherently an autosomal recessive disease. 98% of the mutations that cause this disease occur in the Connexin 26 gene (GJB2).
+ Kit can detect three mutations on this gene; 35DelG, R184P (G> C) and IVS 1 + 1 G> A.
- PRINCIPLE OF THE SYSTEM
+ During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end
+ Separates the reporter dye from the quencer dye only when the probe hybridizes perfectly to the target DNA.
+ This cleavage results in the fluorescent signal which is monitored by Real-Time PCR detection system.
+ An increase in the fluorescent signal (CT) is proportional to the amount of the specific PCR product.
