- PRODUCT DESCRIPTION
+ A recent publication described a new mutation: FV Cambridge.
+ Normally Factor V a (FVa) is associated with venous thrombosis and is resistance to activated C.
+ This inactivation is due to an initial cleavage of the peptide bond on the carboxy side of Arg506 followed by a second cleavage at Arg306.
+ FV Cambridge is a mutation leading to substitution of Arg306 with Thr (Arg306-Thr).
+ This mutation at the 306 site was recreated in recombinant systems.
- PRINCIPLE OF THE SYSTEM
+ During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end
+ Separates the reporter dye from the quencer dye only when the probe hybridizes perfectly to the target DNA.
+ This cleavage results in the fluorescent signal which is monitored by Real-Time PCR detection system.
+ An increase in the fluorescent signal (CT) is proportional to the amount of the specific PCR product.
