- PRODUCT DESCRIPTION
+ Prothrombin is the precursor of the thrombin, a serine protease,
+ Which is a key enzyme in the process of haemostasis and thrombosis with procoagulant, anticoagulant and anti fibrinolytic effects.
+ The polymorphism in the prothrombin gene has been associated with increased risk for venous thrombosis.
+ This polymorphism is an autosomal dominant mutation involving a single base pair substitution,
+ A guanine to adenine substitution at position 20210 in the 3' untranslated region of the prothrombin gene (G20210A).
- PRINCIPLE OF THE SYSTEM
+ During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end
+ Separates the reporter dye from the quencer dye only when the probe hybridizes perfectly to the target DNA.
+ This cleavage results in the fluorescent signal which is monitored by Real-Time PCR detection system.
+ An increase in the fluorescent signal (CT) is proportional to the amount of the specific PCR product.
