- PRODUCT DESCRIPTION
+ Familial Mediterranean Fever (FMF) is an autosomal recessive disorder characterized by recurrent attacks of fever and polyserositis.
+ It affects primarly people of Mediterranean, mostly non-Ashkenazi Jews, Araps and Turks.
+ Kit analysis thirteen mutations, which has been identified in exon 2; E148Q, E148V, in exon 3; P369S, in exon 5; F479L and in exon 10; M680I (G/C-A), I692DEL, M694I, M694V, K695R, V726A, A744S, R761H.
+ Kit is covering 97% mutation rate of FMF in the Anatolian, Middle East countries and many other countries.
- PRINCIPLE OF THE SYSTEM
+ During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencer dye only when the probe hybridizes perfectly to the target DNA.
+ This cleavage results in the fluorescent signal which is monitored by Real-Time PCR detection system.
+ An increase in the fluorescent signal (CT) is proportional to the amount of the specific PCR product.
